n Vitro Fertilization - IVF was developed more than 30 years ago and has since been the most common ART (Assisted Reproduction Technique). It was initially considered a treatment of choice for tubal factor infertility but subsequently expanded to include PCOS (Polycystic Ovarian Syndrome). Today IVF is used for unexplained infertility, advancing maternal age, repeated trials of conservative treatments, ovarian failure, and anti-sperm antibodies. This is a sequential process, requiring harvest of oocytes from the woman’s ovary and receiving ovarian stimulation through medication. Stimulation is given during menstrual cycles to yield more eggs and increase the chances for fertilization. It is monitored carefully via ultrasound and blood tests. Approximately 9 to 14 days into the cycle, Ovum Pick Up (OPU) is performed under general anesthesia. Egg - Oocyte collection is followed by sperm collection. Then the eggs are placed in a special medium where sperms are introduced to induce fertilization.